APPENDIX 4I Digestion of DNA with Restriction Endonucleases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Bloch, K. D. 2001. Digestion of DNA with Restriction Endonucleases. Current Protocols in Protein Science. 13:4I:A.4I.1–A.4I.3.
- Published Online: 1 MAY 2001
- Published Print: SEP 1998
Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. This appendix describes restriction endonucleases and their properties.