Appendix

APPENDIX 4J The Polymerase Chain Reaction

  1. Martha F. Kramer,
  2. Donald M. Coen

Published Online: 1 NOV 2002

DOI: 10.1002/0471140864.psa04js29

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Kramer, M. F. and Coen, D. M. 2002. The Polymerase Chain Reaction. Current Protocols in Protein Science. 29:4J:A.4J.1–A.4J.8.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2002
  2. Published Print: SEP 2002

Abstract

This appendix describes the methodology behind PCR and gives a method for amplifying DNA enzymatically by the PCR and for optimizing this reaction for the sequence and primer set of interest.