Quantitation of Nucleic Acids with Absorption Spectroscopy

Gallagher, Sean

doi:10.1002/0471140864.psa04ks13

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Appendix

APPENDIX 4K Quantitation of Nucleic Acids with Absorption Spectroscopy

Sean Gallagher

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.psa04ks13

Lab Protocol Title

Current Protocols in Protein Science

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How to Cite

Gallagher, S. 2001. Quantitation of Nucleic Acids with Absorption Spectroscopy. Current Protocols in Protein Science. 13:4K:A.4K.1–A.4K.3.

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Motorola Corporation, Tempe, Arizona

Publication History

Published Online: 1 MAY 2001
Published Print: SEP 1998

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Abstract

This appendix describes a protocol for measuring absorption of nucleic acid samples at several different wavelengths to assess purity and concentration. A₂₆₀ measurements are quantitative for relatively pure nucleic acid preparations in microgram quantities. Absorbance readings cannot discriminate between DNA and RNA; however, the ratio of A at 260 and 280 nm can be used as an indicator of nucleic acid purity.