APPENDIX 4L Growth and Manipulation of Yeast

  1. Douglas A. Treco1,
  2. Ann Reynolds2,
  3. Victoria Lundblad3

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.psa04ls14

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Treco, D. A., Reynolds, A. and Lundblad, V. 2001. Growth and Manipulation of Yeast. Current Protocols in Protein Science. 14:4L:A.4L.1–A.4L.6.

Author Information

  1. 1

    Massachusetts General Hospital, Boston, Massachusetts

  2. 2

    University of Washington, Seattle, Washington

  3. 3

    Baylor College of Medicine, Houston, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1998


This unit describes preparation of selected media for growing yeast and also discusses strain storage and revival. Protocols are provided for the assay of β-galactosidase in liquid culture and for transformation using lithium acetate.