UNIT 1.4 Characterization of Opioid Receptors

  1. Robert N. DeHaven,
  2. Diane L. DeHaven-Hudkins

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph0104s08

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

DeHaven, R. N. and DeHaven-Hudkins, D. L. 2001. Characterization of Opioid Receptors. Current Protocols in Pharmacology. 1:1.4.

Author Information

  1. Adolor Corporation, Malvern, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 2000

This is not the most recent version of the article. View current version (1 JUL 2005)


This unit presents two convenient radioligand binding assay methods for opioid receptors. These are applicable to all three of the opioid receptors that have been cloned to date (the mu, kappa and delta receptors), and can be used with numerous commercially available radiolabeled ligands. Moreover, they can serve as reasonable starting points in the development of new assays. A method for determining the binding of radioligand to cloned opioid receptors expressed on the surface of cultured cells is detailed along with a similar method to study radioligand binding to receptors from tissue homogenates. Also included is a procedure for titrating inhibitors of opioid receptor-ligand binding, along with guidelines for analysis of the resulting data.