UNIT 1.8 Radioligand Binding Characterization of Neuronal Nicotinic Acetylcholine Receptors

  1. David J. Anderson

Published Online: 1 DEC 2008

DOI: 10.1002/0471141755.ph0108s43

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Anderson, D. J. 2008. Radioligand Binding Characterization of Neuronal Nicotinic Acetylcholine Receptors. Current Protocols in Pharmacology. 43:1.8:1.8.1–1.8.15.

Author Information

  1. Abbott Laboratories, Abbott Park, Illinois

Publication History

  1. Published Online: 1 DEC 2008
  2. Published Print: DEC 2008


Described in this unit are radioligand binding protocols for three neuronal nicotinic acetylcholine receptor (nAChR) subtypes. Detailed descriptions of binding protocols are presented for the two predominant CNS subtypes of nAChRs, α4β2 and α7, as well as the ganglionic α3β4 nAChR. [3H]Cytisine is utilized for α4β2 nAChRs, while [3H]methyllycaconitine is utilized for α7 nAChRs, both in rat brain. α3β4 nAChRs in IMR-32 cells are labeled with [3H]epibatidine. Reference data are presented, as well as commentary on the current state of neuronal nicotinic receptor research. Curr. Protoc. Pharmacol. 43:1.8.1-1.8.15. © 2008 by John Wiley & Sons, Inc.


  • nicotinic acetylcholine receptors;
  • neuronal nicotinic receptors;
  • nicotinic receptor binding;
  • cytisine;
  • methyllycaconitine;
  • epibatidine;
  • nicotine