UNIT 1.9 Characterization of P1 (Adenosine) Purinoceptors
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Jarvis, M. F. 2001. Characterization of P1 (Adenosine) Purinoceptors. Current Protocols in Pharmacology. 00:1.9:1.9.1–1.9.15.
- Published Online: 1 MAY 2001
- Published Print: MAR 1998
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The purine nucleoside adenosine (ADO) is an important modulator of cellular function in mammalian tissues. ADO modulates neuronal excitability through an interaction with different cell surface receptor subtypes that are heterogeneously distributed in the mammalian central nervous system, as well as in many peripheral tissues. Four different ADO receptor subtypes have been cloned and characterized. Described in this unit are three radioligand binding assays for pharmacological characterization of the high-affinity ADO receptor subtypes A1, A2A, and A3 receptors. Pharmacological characterization of the low-affinity A2B receptor using radioligand binding has been limited by the paucity of potent and selective ligands for this subtype. Since receptor localization is an important criterion for differentiation of receptor subtypes, a support protocol that describes the methodology for the localization of ADO receptors in rat brain tissue using autoradiography is also included.