UNIT 1.12 Characterization of Cholecystokinin (CCK) Receptors

  1. Laurence J. Miller

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph0112s01

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Miller, L. J. 2001. Characterization of Cholecystokinin (CCK) Receptors. Current Protocols in Pharmacology. 1:1.12:1.12.1–1.12.10.

Author Information

  1. Mayo Clinic Center for Basic Research in Digestive Diseases, Rochester, Minnesota

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1998


Cholecystokinin (CCK) and gastrin are structurally related peptide hormones synthesized and secreted by endocrine cells in the gastric antrum, the proximal small intestine, and the central and enteric nervous systems. A broad spectrum of cells express receptors and represent physiologic targets for these peptides. The major physiologic targets for gastrin in the oxyntic gastric mucosa, including the parietal cells and select enterochromaffin cells, while CCK has a broader range of targets, with most involved in nutrient assimilation and appetite control, and the regulation of anxiety. This unit describes binding assays for the determination of receptor number, ligand affinity, and inhibition constants for both CCKA and CCKB receptors, in addition to procedures detailing the preparation of tissues from various sources.