UNIT 1.18 Characterization of the Picrotoxin Site of GABAA Receptors

  1. Ashok K. Mehta,
  2. Maharaj K. Ticku

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph0118s08

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Mehta, A. K. and Ticku, M. K. 2001. Characterization of the Picrotoxin Site of GABAA Receptors. Current Protocols in Pharmacology. 8:1.18:1.18.1–1.18.17.

Author Information

  1. The University of Texas Health Science Center, San Antonio, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 2000

This is not the most recent version of the article. View current version (2 DEC 2013)


This protocol describes an in vitro assay for characterization of the picrotoxin site of GABAA receptors in rat brain membranes using various radioligands. Methods and representative data for Scatchard analysis (Kd, Bmax determination), association kinetics, dissociation kinetics and competition assays (IC50, Ki determination) are included in the protocol.