UNIT 1.18 Characterization of the Picrotoxin Site of GABAA Receptors
Published Online: 2 DEC 2013
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Mehta, A. K. and Ticku, M. K. 2013. Characterization of the Picrotoxin Site of GABAA Receptors. Current Protocols in Pharmacology. 63:1.18:1.18.1–1.18.18.
- Published Online: 2 DEC 2013
This unit describes an in vitro assay for characterization of the picrotoxin site of GABAA receptors in rat brain membranes using various radioligands. Methods and representative data for Scatchard analysis (Kd, Bmax determination), association kinetics, dissociation kinetics, and competition assays (IC50, Ki determination) are included. Curr. Protoc. Pharmacol. 63:1.18.1-1.18.18. © 2013 by John Wiley & Sons, Inc.
- GABAA receptor;
- picrotoxin binding site;
- allosteric regulation;