Unit

UNIT 1.18 Characterization of the Picrotoxin Site of GABAA Receptors

  1. Ashok K. Mehta,
  2. Maharaj K. Ticku

Published Online: 2 DEC 2013

DOI: 10.1002/0471141755.ph0118s63

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Mehta, A. K. and Ticku, M. K. 2013. Characterization of the Picrotoxin Site of GABAA Receptors. Current Protocols in Pharmacology. 63:1.18:1.18.1–1.18.18.

Author Information

  1. The University of Texas Health Science Center, San Antonio, Texas

Publication History

  1. Published Online: 2 DEC 2013

Abstract

This unit describes an in vitro assay for characterization of the picrotoxin site of GABAA receptors in rat brain membranes using various radioligands. Methods and representative data for Scatchard analysis (Kd, Bmax determination), association kinetics, dissociation kinetics, and competition assays (IC50, Ki determination) are included. Curr. Protoc. Pharmacol. 63:1.18.1-1.18.18. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • GABAA receptor;
  • picrotoxin binding site;
  • allosteric regulation;
  • convulsants