UNIT 1.25 Characterization of Calcium Channel Binding
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Zheng, W. 2001. Characterization of Calcium Channel Binding. Current Protocols in Pharmacology. 14:1.25:1.25.1–1.25.12.
- Published Online: 1 NOV 2001
- Published Print: SEP 2001
Voltage-dependent calcium channels are expressed in a variety of tissues including heart, muscles and brain. Saturation binding of a radioligand to the calcium channel is commonly used to characterize the expression level of the channel protein. Compound competition binding assay is a conventional screening method to determine the affinity of unlabeled compounds for the channel protein. This unit provides detailed experimental methods for two types of radioligand binding assays using [3H]PN200-100 and [125I](conotoxin MVIIA. Voltage-dependent calcium channels are expressed in a variety of tissues including heart, muscles and brain