Unit

UNIT 1.27 Radioligand Binding Assays for the Glycine Site on N-Methyl-D-Aspartate Receptors

  1. Scott Perschke1,
  2. John Ferkany2

Published Online: 1 AUG 2002

DOI: 10.1002/0471141755.ph0127s17

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Perschke, S. and Ferkany, J. 2002. Radioligand Binding Assays for the Glycine Site on N-Methyl-D-Aspartate Receptors. Current Protocols in Pharmacology. 17:1.27:1.27.1–1.27.9.

Author Information

  1. 1

    NovaScreen Bioscience Corporation, Hanover, Maryland

  2. 2

    Virtual Drug Discovery, Inc., Avon, Connecticut

Publication History

  1. Published Online: 1 AUG 2002
  2. Published Print: JUN 2002

Abstract

This unit describes a competitive binding assay for the glycine binding site on the NMDA subtype of glutamate receptors in rat brain homogenates. Agonists of the NMDA receptor associated glycine binding site have been proposed as potential therapeutics in cognitive disorders. Conversely, antagonists may be useful in a variety of disorders associated with excessive activation of EAA receptors, including Parkinsons, Huntingtons and Alzheimers Diseases, and neuropathic pain, among others. This unit describes a competitive binding assay for the glycine binding site on the NMDA subtype of glutamate receptors in rat