Unit

UNIT 1.28 Characterization of Melanocortin Receptors

  1. Aaron S. Goetz,
  2. Diane M. Ignar

Published Online: 1 NOV 2003

DOI: 10.1002/0471141755.ph0128s22

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Goetz, A. S. and Ignar, D. M. 2003. Characterization of Melanocortin Receptors. Current Protocols in Pharmacology. 22:1.28:1.28.1–1.28.8.

Author Information

  1. GlaxoSmithKline, Research Triangle Park, North Carolina

Publication History

  1. Published Online: 1 NOV 2003
  2. Published Print: SEP 2003

Abstract

This unit describes a Scintillation Proximity Assay (SPA) for the measurement of ligand binding to melanocortin receptors (MCRs) using membranes prepared from cell lines stably expressing recombinant MCRs. It provides a facile method for determining the affinity of compounds at MC1R, MC3R, MC4R, or MC5R.

Keywords:

  • melanocortins;
  • melanocortin receptors;
  • SPA;
  • MC1R;
  • MC3R;
  • MC4R;
  • MC5R;
  • NDP-α-MSH;
  • MSH