UNIT 1.29 Characterization of Neurotensin Receptors
Published Online: 1 MAY 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Kitabgi, P. and Pélaprat, D. 2004. Characterization of Neurotensin Receptors. Current Protocols in Pharmacology. 24:1.29.1–1.29.16.
- Published Online: 1 MAY 2004
- Published Print: MAR 2004
This unit describes procedures for performing competition binding assays with neurotensin receptor subtypes 1 and 2 (NTS1 and NTS2). Binding assays using cloned receptors, brain membranes, and primary cultured mesencephalic neurons are presented. NTS1 binding assays employing either radiolabeled neurotensin or SR 48692 (a nonpeptide neurotensin antagonist) as radioligands are described. These procedures may be used to screen selective ligands at neurotensin receptor subtypes.
- SR 48692;
- neurotensin receptors;
- binding assay;
- brain membranes;
- mesencephalic neurons