Unit

UNIT 1.29 Characterization of Neurotensin Receptors

  1. Patrick Kitabgi,
  2. Didier Pélaprat

Published Online: 1 MAY 2004

DOI: 10.1002/0471141755.ph0129s24

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Kitabgi, P. and Pélaprat, D. 2004. Characterization of Neurotensin Receptors. Current Protocols in Pharmacology. 24:1.29:1.29.1–1.29.16.

Author Information

  1. Hopital St-Antoine, Paris, France

Publication History

  1. Published Online: 1 MAY 2004
  2. Published Print: MAR 2004

Abstract

This unit describes procedures for performing competition binding assays with neurotensin receptor subtypes 1 and 2 (NTS1 and NTS2). Binding assays using cloned receptors, brain membranes, and primary cultured mesencephalic neurons are presented. NTS1 binding assays employing either radiolabeled neurotensin or SR 48692 (a nonpeptide neurotensin antagonist) as radioligands are described. These procedures may be used to screen selective ligands at neurotensin receptor subtypes.

Keywords:

  • neurotensin;
  • SR 48692;
  • neurotensin receptors;
  • NTS1;
  • NTS2;
  • binding assay;
  • brain membranes;
  • mesencephalic neurons