UNIT 2.3 Overview of Phosphoinositide Hydrolysis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Kendall, D. 2001. Overview of Phosphoinositide Hydrolysis. Current Protocols in Pharmacology. 00:2.3:2.3.1–2.3.6.
- Published Online: 1 MAY 2001
- Published Print: MAR 1998
Phosphoinositide hydrolysis is a ubiquitous, multifunctional, intracellular signaling mechanism induced (via receptor activation) by a wide variety of signaling molecules such as hormones and neurotransmitters. Phosphoinositide hydrolysis generates intracellular second messengers that regulate cell function. This overview describes the major molecular players involved in phosphoinositide signaling along with experimental strategies for measuring phosphoinositide hydrolysis, including inositol radiolabeling methodologies, membrane phospholipase C assays, cytidine prelabeling, and mass measurements of phosphoinositide cycle products.