UNIT 2.3 Overview of Phosphoinositide Hydrolysis

  1. David Kendall

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph0203s00

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Kendall, D. 2001. Overview of Phosphoinositide Hydrolysis. Current Protocols in Pharmacology. 00:2.3:2.3.1–2.3.6.

Author Information

  1. University of Nottingham Medical School, Nottingham, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1998


Phosphoinositide hydrolysis is a ubiquitous, multifunctional, intracellular signaling mechanism induced (via receptor activation) by a wide variety of signaling molecules such as hormones and neurotransmitters. Phosphoinositide hydrolysis generates intracellular second messengers that regulate cell function. This overview describes the major molecular players involved in phosphoinositide signaling along with experimental strategies for measuring phosphoinositide hydrolysis, including inositol radiolabeling methodologies, membrane phospholipase C assays, cytidine prelabeling, and mass measurements of phosphoinositide cycle products.