UNIT 3.9 Cytochrome P450 Assays
Published Online: 1 FEB 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Delaporte, E. and Rodrigues, A. D. 2002. Cytochrome P450 Assays. Current Protocols in Pharmacology. 15:3.9:3.9.1–3.9.32.
- Published Online: 1 FEB 2002
- Published Print: DEC 2001
Cytochrome P450s (CYPs) play a major role in drug detoxification, and inhibition of CYP-mediated metabolism may lead to accumulation of toxic drug levels in the plasma. To prevent adverse drug-drug interactions, new drug candidates are routinely tested for their ability to inhibit these enzymes. This unit describes a variety of protocols for evaluating new chemical entities as inhibitors of CYP activity. An example protocol illustrates a high-throughput screening format using a fluorogenic probe, and a method for evaluating a test compound as a time-dependent inhibitor of CYP is also described.