UNIT 4.5 α-Adrenoceptor Assays

  1. Peter W. Abel,
  2. Neha Jain

Published Online: 1 DEC 2012

DOI: 10.1002/0471141755.ph0405s59

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Abel, P. W. and Jain, N. 2012. α-Adrenoceptor Assays. Current Protocols in Pharmacology. 59:4.5:4.5.1–4.5.32.

Author Information

  1. Department of Pharmacology, Creighton University School of Medicine, Omaha, Nebraska

Publication History

  1. Published Online: 1 DEC 2012


α-Adrenoceptors mediate responses to activation of both peripheral sympathetic nerves and central noradrenergic neurons. They also serve as autoreceptors that modulate the release of norepinephrine (NE) and other neurotransmitters. There are two major classes of α-adrenoceptors, the α1- and α2. Each class is subdivided into three subtypes: α1A, α1B, α1D, and α2A, α2B, α2C. Described in this unit are in vitro isolated tissue methods used to study α-adrenoceptor functions and to identify novel ligands for these receptors. Detailed protocols describing use of isolated tissues to study the various α1- and α2-adrenoceptor subtypes are provided. Curr. Protoc. Pharmacol. 59:4.5.1-4.5.32. © 2012 by John Wiley & Sons, Inc.


  • alpha adrenoceptors;
  • bioassay;
  • isolated tissues