UNIT 4.10 Tachykinin Receptor Assays
Published Online: 1 MAR 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Meini, S. and Alberto Maggi, C. 2010. Tachykinin Receptor Assays. Current Protocols in Pharmacology. 48:4.10:4.10.1–4.10.28.
- Published Online: 1 MAR 2010
- Published Print: MAR 2010
Described in this unit are methods for obtaining, preparing, and testing smooth muscle preparations bearing tachykinin receptors to study the agonist or antagonist properties of test compounds. Concentration-response curves to agonists are constructed to measure their ability to produce smooth muscle contractions and thus evaluate the potency and efficacy of the agonists. Antagonists are tested for their ability to shift the agonist concentration-response curve and to calculate their potency. Two different protocols are described for each of the three tachykinin receptors (NK1, NK2, and NK3). The NK1 receptor assays use guinea pig ileum longitudinal muscle myenteric plexus (GPI) and rat urinary bladder (RUB), the NK2 receptor assays use isolated endothelium-deprived rabbit pulmonary artery (RPA) and hamster trachea (HT), and the NK3 receptor assays use GPI and rat portal vein (RPV). Curr. Protoc. Pharmacol. 48:4.10.1-4.10.28. © 2010 by John Wiley & Sons, Inc.
- tachykinin receptor;
- smooth muscle;
- in vitro bioassay;