Unit

UNIT 4.10 Tachykinin Receptor Assays

  1. Stefania Meini,
  2. Carlo Alberto Maggi

Published Online: 1 MAR 2010

DOI: 10.1002/0471141755.ph0410s48

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Meini, S. and Alberto Maggi, C. 2010. Tachykinin Receptor Assays. Current Protocols in Pharmacology. 48:4.10:4.10.1–4.10.28.

Author Information

  1. Menarini Ricerche, Florence, Italy

Publication History

  1. Published Online: 1 MAR 2010
  2. Published Print: MAR 2010

Abstract

Described in this unit are methods for obtaining, preparing, and testing smooth muscle preparations bearing tachykinin receptors to study the agonist or antagonist properties of test compounds. Concentration-response curves to agonists are constructed to measure their ability to produce smooth muscle contractions and thus evaluate the potency and efficacy of the agonists. Antagonists are tested for their ability to shift the agonist concentration-response curve and to calculate their potency. Two different protocols are described for each of the three tachykinin receptors (NK1, NK2, and NK3). The NK1 receptor assays use guinea pig ileum longitudinal muscle myenteric plexus (GPI) and rat urinary bladder (RUB), the NK2 receptor assays use isolated endothelium-deprived rabbit pulmonary artery (RPA) and hamster trachea (HT), and the NK3 receptor assays use GPI and rat portal vein (RPV). Curr. Protoc. Pharmacol. 48:4.10.1-4.10.28. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • tachykinin receptor;
  • smooth muscle;
  • contractility;
  • antagonism;
  • agonism;
  • in vitro bioassay;
  • selectivity