UNIT 4.14 Dopamine 2 (DA-2) Receptor Assays Using Sympathetic Nerve Terminals

  1. John P. Long

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph0414s06

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Long, J. P. 2001. Dopamine 2 (DA-2) Receptor Assays Using Sympathetic Nerve Terminals. Current Protocols in Pharmacology. 6:4.14:4.14.1–4.14.12.

Author Information

  1. University of Iowa, Iowa City, Iowa

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1999


In vitro experiments using isolated atria with and without superfusion can be used to quantify the dopamine-2 (DA-2) receptor stimulating activity of compounds. This receptor mechanism decreases the amount of norepinephrine released from sympathetic nerve terminals by neuronal impulses. DA-2 receptor agonists (stimulants) are very active and induce several physiological changes, such as bradycardia, hypotension, lower intraocular pressure, etc. In this unit, tissue preparation and dopamine receptor assays are described for isolated cat atria with field stimulation or with superfusion, and isolated guinea pig atria with field stimulation.