UNIT 4.14 Dopamine 2 (DA-2) Receptor Assays Using Sympathetic Nerve Terminals
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Long, J. P. 2001. Dopamine 2 (DA-2) Receptor Assays Using Sympathetic Nerve Terminals. Current Protocols in Pharmacology. 6:4.14:4.14.1–4.14.12.
- Published Online: 1 MAY 2001
- Published Print: SEP 1999
In vitro experiments using isolated atria with and without superfusion can be used to quantify the dopamine-2 (DA-2) receptor stimulating activity of compounds. This receptor mechanism decreases the amount of norepinephrine released from sympathetic nerve terminals by neuronal impulses. DA-2 receptor agonists (stimulants) are very active and induce several physiological changes, such as bradycardia, hypotension, lower intraocular pressure, etc. In this unit, tissue preparation and dopamine receptor assays are described for isolated cat atria with field stimulation or with superfusion, and isolated guinea pig atria with field stimulation.