UNIT 4.19 5-Hydroxytryptamine Receptor Assays

  1. Katherine W. Figueroa1,
  2. Graeme R. Martin2,
  3. M. Teresa Pulido-Rios3

Published Online: 1 SEP 2009

DOI: 10.1002/0471141755.ph0419s46

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Figueroa, K. W., Martin, G. R. and Pulido-Rios, M. T. 2009. 5-Hydroxytryptamine Receptor Assays. Current Protocols in Pharmacology. 46:4.19:4.19.1–4.19.45.

Author Information

  1. 1

    Johnson & Johnson PRD, La Jolla, California

  2. 2

    Takeda Research Investment, Palo Alto, California

  3. 3

    Theravance, South San Francisco, California

Publication History

  1. Published Online: 1 SEP 2009
  2. Published Print: SEP 2009


5-Hydroxytryptamine (5-HT) receptors, by virtue of their broad expression pattern in peripheral and central tissues, regulate diverse physiological and behavioral responses through the activation of fourteen molecularly distinct receptor subtypes. The tissue-specific distribution of these receptors confers specificity for the actions of serotonin and highlights the therapeutic potential of serotonin receptor modulators. To better assess this therapeutic potential, it is useful to characterize serotonergic agonists and antagonists in physiologically relevant organ systems. Provided in this unit are twelve tissue bath assays using vascular and smooth muscle tissues isolated from guinea-pig, rat, and rabbit. These tests make possible the analyses of compounds at nine serotonin receptor subtypes. Curr. Protoc. Pharmacol. 46:4.19.1-4.19.45. © 2009 by John Wiley & Sons, Inc.


  • serotonin;
  • 5-hydroxytriptamine;
  • 5-HT