UNIT 4.19 5-Hydroxytryptamine Receptor Assays
Published Online: 1 SEP 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Figueroa, K. W., Martin, G. R. and Pulido-Rios, M. T. 2009. 5-Hydroxytryptamine Receptor Assays. Current Protocols in Pharmacology. 46:4.19:4.19.1–4.19.45.
- Published Online: 1 SEP 2009
- Published Print: SEP 2009
5-Hydroxytryptamine (5-HT) receptors, by virtue of their broad expression pattern in peripheral and central tissues, regulate diverse physiological and behavioral responses through the activation of fourteen molecularly distinct receptor subtypes. The tissue-specific distribution of these receptors confers specificity for the actions of serotonin and highlights the therapeutic potential of serotonin receptor modulators. To better assess this therapeutic potential, it is useful to characterize serotonergic agonists and antagonists in physiologically relevant organ systems. Provided in this unit are twelve tissue bath assays using vascular and smooth muscle tissues isolated from guinea-pig, rat, and rabbit. These tests make possible the analyses of compounds at nine serotonin receptor subtypes. Curr. Protoc. Pharmacol. 46:4.19.1-4.19.45. © 2009 by John Wiley & Sons, Inc.