UNIT 5.16 Models of Anxiety: Stress-Induced Hyperthermia (SIH) in Singly Housed Mice

  1. Lucianne Groenink1,
  2. Christiaan Vinkers1,
  3. Ruud van Oorschot1,
  4. Berend Olivier1,2,3

Published Online: 1 JUN 2009

DOI: 10.1002/0471141755.ph0516s45

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Groenink, L., Vinkers, C., van Oorschot, R. and Olivier, B. 2009. Models of Anxiety: Stress-Induced Hyperthermia (SIH) in Singly Housed Mice. Current Protocols in Pharmacology. 45:5.16:5.16.1–5.16.12.

Author Information

  1. 1

    Utrecht Institute for Pharmaceutical Sciences and Rudolf Magnus Institute of Neuroscience, Utrecht, The Netherlands

  2. 2

    PsychoGenics Inc., Tarrytown, New York

  3. 3

    Yale University School of Medicine, New Haven, Connecticut

Publication History

  1. Published Online: 1 JUN 2009
  2. Published Print: JUN 2009


Described in this unit is the stress-induced hyperthermia (SIH) test in mice in a single-housed format. This protocol has proven reliable in detecting the anxiolytic properties of test compounds. In this test, SIH is quantified in singly housed mice using a rectal temperature measurement as the stressor. Rectal temperature is measured twice at a 10-min interval. Due to the stress experienced during the first temperature measurement, the temperature of the second measurement (T2) is ∼0.8° to 1.5°C higher than that of the first (T1). This difference in temperature (ΔT = T2T1) is defined as the SIH response. The SIH response is reduced by different classes of anxiolytics. The SIH test is simple and robust, it does not require training of animals, and test compound effects on motor behavior, feeding, and nociception do not affect test outcome. Furthermore, it is one of few anxiety tests that focuses on the physiological component of anxiety. Curr. Protoc. Pharmacol. 45:5.16.1-5.16.12. © 2009 by John Wiley & Sons, Inc.


  • stress;
  • hyperthermia;
  • body temperature;
  • mouse;
  • anxiety