UNIT 6.1 Overview of Receptor Cloning
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Nash, N. 2001. Overview of Receptor Cloning. Current Protocols in Pharmacology. 00:6.1.1–6.1.12.
- Published Online: 1 MAY 2001
- Published Print: MAR 1998
Receptors are generally categorized into four major superfamilies according to their structural and genetic similarities: steroid receptors, growth factor receptors, ionotropic receptors (i.e., ligand-gated ion channels), and metabotropic receptors (i.e., G protein-coupled receptors). This unit describes the methodology and critical issues for cloning receptors by several methods. Although cloning by protein purification was the first major method, it is now less frequently employed. The early, more tedious procedures broadened knowledge about receptors and established a strong foundation that has enabled the utilization of the newer approaches discussed in this unit, including: homology cloning by screening with oligonucleotides, DNA fragments or the products of direct polymerase chain reaction (PCR); expression cloning using function, ligand binding, and antibody recognition; and differential display techniques.