Unit

UNIT 6.3 Expression of Cloned Receptors in Mammalian Cell Lines

  1. Murali Gopalakrishnan,
  2. Eduardo J. Molinari

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph0603s00

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Gopalakrishnan, M. and Molinari, E. J. 2001. Expression of Cloned Receptors in Mammalian Cell Lines. Current Protocols in Pharmacology. 00:6.3:6.3.1–6.3.13.

Author Information

  1. Abbott Laboratories, Abbott Park, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1998

Abstract

The expression of cloned receptor and ion-channel proteins is a very useful tool for studying the physiological and pharmacological properties of receptors. The process of introducing a gene of interest into eukaryotic cells by biochemical or physical methods is termed transfection. After transfection, some of the DNA is transported into the nucleus, where it is transcribed. The resulting mRNA is transferred into the cytoplasm and translated into protein. The transfection method described in this unit is a versatile, plasmid-based, liposome-mediated gene-transfer technique known as lipofection. This technique has gained wide acceptance because of its simplicity, suitability for a wide range of cell types, reasonable transfection efficiencies, and reproducibility. This unit also includes a procedure for assessing transfection efficiency via cotransfection with a reporter gene (lacZ) and assay for coexpression.