UNIT 7.1 Overview of Pharmacokinetics

  1. Lisa D. Aimone

Published Online: 1 OCT 2005

DOI: 10.1002/0471141755.ph0701s30

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Aimone, L. D. 2005. Overview of Pharmacokinetics. Current Protocols in Pharmacology. 30:7.1:7.1.1–7.1.26.

Author Information

  1. Cephalon, Inc., West Chester, Pennsylvania

Publication History

  1. Published Online: 1 OCT 2005
  2. Published Print: SEP 2005

This is not the most recent version of the article. View current version (2 SEP 2014)


In addition to having potency against the molecular target, a compound must be able to reach its intended site of action in vivo in sufficient quantity and for the appropriate duration to exert a biological effect. The fate of a compound after in vivo administration depends upon the absorption, distribution, metabolism, and excretion (ADME) of the compound, and the concentration of the compound in the blood, plasma, or other tissues represents the sum of all of these processes. Described in this unit are protocols for administering a compound by a variety of routes and for collecting the appropriate samples to determine the pharmacokinetic profile. The basic terms used in pharmacokinetic studies are defined, and representative examples are given to illustrate important variables to consider.


  • Pharmacokinetic parameters;
  • In vivo routes of administration;
  • blood and tissue collection;
  • bioavailability;
  • half-life;
  • mass balance;
  • rat