Unit

UNIT 7.2 Estimating Intestinal Mucosal Permeation of Compounds Using Caco-2 Cell Monolayers

  1. Jinnian Gao,
  2. Erin D. Hugger,
  3. Melissa S. Beck-Westermeyer,
  4. Ronald T. Borchardt

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph0702s08

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Gao, J., Hugger, E. D., Beck-Westermeyer, M. S. and Borchardt, R. T. 2001. Estimating Intestinal Mucosal Permeation of Compounds Using Caco-2 Cell Monolayers. Current Protocols in Pharmacology. 8:7.2:7.2.1–7.2.23.

Author Information

  1. University of Kansas, Lawrence, Kansas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 2000

Abstract

Step-by-step protocols are provided in this unit for the measurement of apparent permeability coefficients of compounds using Caco-2 cell monolayers as an in vitro model of the intestinal mucosa. Procedures for culturing the cells and transmonolayer transport studies are also included. Critical issues for successfully estimating intestinal mucosal permeation of drugs are discussed. Step-by-step protocols are provided in this unit for the measurement of apparent permeability coefficients of compounds using