UNIT 7.4 In Vivo Measurement of Blood-Brain Barrier Permeability
Published Online: 1 AUG 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Boje, K. M. 2002. In Vivo Measurement of Blood-Brain Barrier Permeability. Current Protocols in Pharmacology. 17:7.4:7.4.1–7.4.39.
- Published Online: 1 AUG 2002
- Published Print: JUN 2002
This unit describes various protocols for the in vivo quantitation of drug permeability across the rodent blood-brain barrier. Methods for the measurement of drug influx and efflux are described, and support protocols are provided for determining intravascular capillary volume and cerebral perfusion flow. An in situ perfusion technique is also provided for assessing whether transport of a test compound occurs by carrier-mediated or saturable transport.