UNIT 7.12 The Ussing Chamber and Measurement of Drug Actions on Mucosal Ion Transport
Published Online: 1 JUN 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Brown, D. R. and O’Grady, S. M. 2008. The Ussing Chamber and Measurement of Drug Actions on Mucosal Ion Transport. Current Protocols in Pharmacology. 41:7.12:7.12.1–7.12.17.
- Published Online: 1 JUN 2008
- Published Print: JUN 2008
Ussing chamber technology can be used to investigate the pharmacodynamic and pharmacokinetic properties of drugs and natural substances on polarized epithelia. With this approach, epithelial sheets or confluent epithelial cell monolayers are mounted between two half-chambers, permitting compound delivery to either the apical or basolateral cell surface. Described in this unit are protocols for determining the effects of test compounds on transepithelial ion transport processes. Curr. Protoc. Pharmacol. 41:7.12.1-7.12.17. © 2008 by John Wiley & Sons, Inc.
- epithelial cell culture;
- transepithelial ion transport;