Unit

UNIT 7.12 The Ussing Chamber and Measurement of Drug Actions on Mucosal Ion Transport

  1. David R. Brown,
  2. Scott M. O’Grady

Published Online: 1 JUN 2008

DOI: 10.1002/0471141755.ph0712s41

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Brown, D. R. and O’Grady, S. M. 2008. The Ussing Chamber and Measurement of Drug Actions on Mucosal Ion Transport. Current Protocols in Pharmacology. 41:7.12:7.12.1–7.12.17.

Author Information

  1. University of Minnesota, Departments of Veterinary & Biomedical Sciences (DRB) and Animal Science (SMO), St. Paul, Minnesota

Publication History

  1. Published Online: 1 JUN 2008
  2. Published Print: JUN 2008

Abstract

Ussing chamber technology can be used to investigate the pharmacodynamic and pharmacokinetic properties of drugs and natural substances on polarized epithelia. With this approach, epithelial sheets or confluent epithelial cell monolayers are mounted between two half-chambers, permitting compound delivery to either the apical or basolateral cell surface. Described in this unit are protocols for determining the effects of test compounds on transepithelial ion transport processes. Curr. Protoc. Pharmacol. 41:7.12.1-7.12.17. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • epithelium;
  • epithelial cell culture;
  • intestine;
  • airways;
  • skin;
  • transepithelial ion transport;
  • pharmacokinetics