UNIT 8.1 In Vitro Autoradiography
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Kuhar, M. J. 2001. In Vitro Autoradiography. Current Protocols in Pharmacology. 00:8.1:8.1.1–8.1.9.
- Published Online: 1 MAY 2001
- Published Print: MAR 1998
The light-microscopic localization of receptors and other molecules by autoradiography of radioligand binding in tissue sections provides a high degree of anatomical resolution and the ability to associate even small quantities of binding sites with anatomical structures. The basic procedure in this unit involves incubating slide-mounted tissue sections with radioligands, washing and drying of the sections with specifically bound ligands under conditions that preserve ligand binding, and visualizing and quantifying the binding sites in the tissues. While the procedures were developed for drug and neurotransmitter receptors, any binding site can be visualized with a sufficiently selective, high-affinity radioligand. If optimal conditions for radioligand binding to sites in slide-mounted tissue sections are already known, the basic protocol in this unit can be used to create autoradiograms. However, if a radioligand is being studied for the first time, guidelines are provided on optimizing experimental parameters for binding, washing, and drying. The light-microscopic localization of receptors and other molecules by autoradiography of radioligand binding in tissue sectio.