Unit

UNIT 8.2 Autoradiographic Localization of Growth Factor Receptors in Neuronal Tissues

  1. Sylvain Doré1,
  2. Satyabrata Kar1,
  3. Daniel Auld1,
  4. Jean-Guy Chabot1,
  5. Remi Quirion2

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph0802s03

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Doré, S., Kar, S., Auld, D., Chabot, J.-G. and Quirion, R. 2001. Autoradiographic Localization of Growth Factor Receptors in Neuronal Tissues. Current Protocols in Pharmacology. 3:8.2:8.2.1–8.2.16.

Author Information

  1. 1

    Douglas Hospital Research Center and McGill University, Montreal, Canada

  2. 2

    Douglas Hospital Research Center, Verdun, Canada

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1998

Abstract

The physiological and pharmacological effects of growth factors, like those of neurotransmitters and neuromodulators, are mediated by binding to specific receptor sites. Using radiolabeled molecules with high specific activity, such as the endogenous ligand itself, it is possible to measure receptor binding in membrane preparations. This technique provides a means to characterize the selectivity and specificity of the receptors for a particular growth factor. Additionally, ligand-binding assays make it possible to study in detail the structure-activity relationships and mechanisms of action of growth factors. As an example of this methodology, this unit describes the use of both film and emulsion autoradiography to study the pharmacological characteristics and cellular distribution profile of insulin-like growth factor receptors in the central nervous system. For the sake of clarity, in vitro film autoradiography in tissue sections and liquid emulsion autoradiography in cultured neurons are discussed separately. The physiological and pharmacological effects of growth factors, like those of neurotransmitters and neuromodulators, are medi.