UNIT 8.2 Autoradiographic Localization of Growth Factor Receptors in Neuronal Tissues
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Doré, S., Kar, S., Auld, D., Chabot, J.-G. and Quirion, R. 2001. Autoradiographic Localization of Growth Factor Receptors in Neuronal Tissues. Current Protocols in Pharmacology. 3:8.2:8.2.1–8.2.16.
- Published Online: 1 MAY 2001
- Published Print: DEC 1998
The physiological and pharmacological effects of growth factors, like those of neurotransmitters and neuromodulators, are mediated by binding to specific receptor sites. Using radiolabeled molecules with high specific activity, such as the endogenous ligand itself, it is possible to measure receptor binding in membrane preparations. This technique provides a means to characterize the selectivity and specificity of the receptors for a particular growth factor. Additionally, ligand-binding assays make it possible to study in detail the structure-activity relationships and mechanisms of action of growth factors. As an example of this methodology, this unit describes the use of both film and emulsion autoradiography to study the pharmacological characteristics and cellular distribution profile of insulin-like growth factor receptors in the central nervous system. For the sake of clarity, in vitro film autoradiography in tissue sections and liquid emulsion autoradiography in cultured neurons are discussed separately. The physiological and pharmacological effects of growth factors, like those of neurotransmitters and neuromodulators, are medi.