Unit

UNIT 9.2 Cell-Based Assays Using the Fluorometric Imaging Plate Reader (FLIPR)

  1. Kristi L. Whiteaker,
  2. James P. Sullivan,
  3. Murali Gopalakrishnan

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph0902s09

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Whiteaker, K. L., Sullivan, J. P. and Gopalakrishnan, M. 2001. Cell-Based Assays Using the Fluorometric Imaging Plate Reader (FLIPR). Current Protocols in Pharmacology. 9:9.2:9.2.1–9.2.23.

Author Information

  1. Abbott Laboratories, Abbott Park, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 2000

Abstract

A critical component of the drug discovery and development process is the identification of novel pharmacophores. Such discovery efforts are, in general, facilitated by rapid and high-throughput cell-based assays of receptor/ion channel-mediated signaling processes to screen diverse chemical libraries of compounds possessing activator or inhibitor activities at the desired target. The availability of the Flurometric Image Plate Reader (FLIPR) has made rapid assays of cellular signaling processes feasible by simultaneous kinetics measurement of cell-based fluorescence changes in a 96- or 384-well format. This unit describes the application of the FLIPR in cell-based kinetic assays for measuring membrane potential changes and intracellular calcium dynamics.