UNIT 9.2 Cell-Based Assays Using the Fluorometric Imaging Plate Reader (FLIPR)
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Whiteaker, K. L., Sullivan, J. P. and Gopalakrishnan, M. 2001. Cell-Based Assays Using the Fluorometric Imaging Plate Reader (FLIPR). Current Protocols in Pharmacology. 9:9.2:9.2.1–9.2.23.
- Published Online: 1 MAY 2001
- Published Print: JUN 2000
A critical component of the drug discovery and development process is the identification of novel pharmacophores. Such discovery efforts are, in general, facilitated by rapid and high-throughput cell-based assays of receptor/ion channel-mediated signaling processes to screen diverse chemical libraries of compounds possessing activator or inhibitor activities at the desired target. The availability of the Flurometric Image Plate Reader (FLIPR) has made rapid assays of cellular signaling processes feasible by simultaneous kinetics measurement of cell-based fluorescence changes in a 96- or 384-well format. This unit describes the application of the FLIPR in cell-based kinetic assays for measuring membrane potential changes and intracellular calcium dynamics.