Unit

UNIT 11.1 Overview of Electrophysiology

  1. Daniel Bertrand

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph1101s03

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Bertrand, D. 2001. Overview of Electrophysiology. Current Protocols in Pharmacology. 3:11.1:11.1.1–11.1.11.

Author Information

  1. University of Geneva, Geneva, Switzerland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1998

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Abstract

This unit provides an overview of the electrophysiological techniques used to determine the physiological and/or pharmacological properties of membrane ion channels at the single-cell level. Only those techniques that measure potential changes at the transmembrane and membrane levels are discussed. The basics of current-clamp recording, voltage-clamp recording, and patch-clamp procedures are presented along with their advantages and disadvantages. Additional topics are discussed with regard to their relation to electrophysiological measurements, such as membrane properties, ion selectivity, time resolution, ligand-gated ion channels (LGICs), voltage-dependent channels, and channel modulation and interactions. Also included is a discussion the electrophysiological properties of seven-transmembrane-domain receptors. This unit provides an overview of the electrophysiological techniques used to determine the physiological and/or pharmacologic of ion channels.