UNIT 11.2 Electrophysiological Analysis of G Protein–Coupled Receptors in Mammalian Neurons

  1. William R. Proctor,
  2. Thomas V. Dunwiddie

Published Online: 1 MAY 2001

DOI: 10.1002/0471141755.ph1102s07

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Proctor, W. R. and Dunwiddie, T. V. 2001. Electrophysiological Analysis of G Protein–Coupled Receptors in Mammalian Neurons. Current Protocols in Pharmacology. 7:11.2:11.2.1–11.2.22.

Author Information

  1. Veterans Administration Medical Research Service, Denver, Colorado

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1999


This unit describes general techniques that are useful for recording electrophysiological responses that are mediated via the activation of G-protein coupled receptors (GPCRs). It includes a brief description of preparations, but focuses primarily on experiments using hippocampal brain slice preparations. Techniques for the preparation of brain slices, electrodes, filling solutions, and the recording protocols that are suitable for assessing the activity of GPCRs using electrophysiological techniques are summarized, and various protocols for the activation of these receptors are discussed.