Unit

UNIT 11.3 The Action Potential of the Purkinje Fiber: An In Vitro Model for Evaluation of the Proarrhythmic Potential of Cardiac and Noncardiac Drugs

  1. René Rouet1,
  2. Joffrey Ducroq1,
  3. Sandra Picard2

Published Online: 1 FEB 2002

DOI: 10.1002/0471141755.ph1103s15

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Rouet, R., Ducroq, J. and Picard, S. 2002. The Action Potential of the Purkinje Fiber: An In Vitro Model for Evaluation of the Proarrhythmic Potential of Cardiac and Noncardiac Drugs. Current Protocols in Pharmacology. 11:11.3.

Author Information

  1. 1

    Université de Caen, Boulogne-Billancourt, France

  2. 2

    Porsolt and Partners Pharmacology, Boulogne-Billancourt, France

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: DEC 2001

This is not the most recent version of the article. View current version (1 JUL 2006)

Abstract

The proarrhythmic potential of new drugs can be investigated using in vitro electrophysiological techniques measuring the cardiac action potential in isolated Purkinje fibers. Different types of arrhythmias may occur, as early after-depolarizations (EADs), which are favored by action potential duration lengthening and bradycardia, or delayed after-depolarizations (DADs), which are facilitated by tachycardia. The effects of a test compound on the occurrence of these arrhythmias, known to induce in vivo torsades de pointes (or triggered activities), can be studied following the experimental protocols described in this unit.