UNIT 11.3 The Action Potential of the Purkinje Fiber: An In Vitro Model for Evaluation of the Proarrhythmic Potential of Cardiac and Noncardiac Drugs
Published Online: 1 FEB 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Rouet, R., Ducroq, J. and Picard, S. 2002. The Action Potential of the Purkinje Fiber: An In Vitro Model for Evaluation of the Proarrhythmic Potential of Cardiac and Noncardiac Drugs. Current Protocols in Pharmacology. 11:11.3.
- Published Online: 1 FEB 2002
- Published Print: DEC 2001
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The proarrhythmic potential of new drugs can be investigated using in vitro electrophysiological techniques measuring the cardiac action potential in isolated Purkinje fibers. Different types of arrhythmias may occur, as early after-depolarizations (EADs), which are favored by action potential duration lengthening and bradycardia, or delayed after-depolarizations (DADs), which are facilitated by tachycardia. The effects of a test compound on the occurrence of these arrhythmias, known to induce in vivo torsades de pointes (or triggered activities), can be studied following the experimental protocols described in this unit.