Unit

UNIT 11.3 The Action Potential of the Purkinje Fiber: An In Vitro Model for Evaluation of the Proarrhythmic Potential of Cardiac and Noncardiac Drugs

  1. Sandra Picard1,
  2. Sonia Goineau1,
  3. Rene Rouet2

Published Online: 1 JUL 2006

DOI: 10.1002/0471141755.ph1103s33

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Picard, S., Goineau, S. and Rouet, R. 2006. The Action Potential of the Purkinje Fiber: An In Vitro Model for Evaluation of the Proarrhythmic Potential of Cardiac and Noncardiac Drugs. Current Protocols in Pharmacology. 33:11.3:11.3.1–11.3.16.

Author Information

  1. 1

    Porsolt and Partners Pharmacology, Boulogne-Billancourt, France

  2. 2

    Universite de Caen, Caen, France

Publication History

  1. Published Online: 1 JUL 2006
  2. Published Print: JUN 2006

Abstract

The proarrhythmic potential of new chemical entities can be investigated using in vitro electrophysiological techniques measuring the cardiac action potential in isolated Purkinje fibers. Different types of arrhythmias may occur as early afterdepolarizations (EADs), which are favored by action potential duration lengthening and bradycardia, or as delayed afterdepolarizations (DADs), which are facilitated by tachycardia. The effects of a test compound on the occurrence of these arrhythmias, thought to be responsible for the development of torsades de pointes in the clinic can be studied using the experimental protocols described in this unit.

Keywords:

  • cardiac risk assessment;
  • proarrhythmic potential;
  • Purkinje fiber;
  • action potential;
  • safety pharmacology