UNIT 11.4 Use of Electrophysiological Methods in the Study of Recombinant and Native Neuronal Ligand-Gated Ion Channels
Published Online: 1 AUG 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Thomas, P. and Smart, T. G. 2002. Use of Electrophysiological Methods in the Study of Recombinant and Native Neuronal Ligand-Gated Ion Channels. Current Protocols in Pharmacology. 17:11.4:11.4.1–11.4.34.
- Published Online: 1 AUG 2002
- Published Print: JUN 2002
This is not the most recent version of the article. View current version (1 DEC 2012)
This unit is geared towards investigators wishing to study the effects of externally and internally applied agents on the behavior of ligand-gated ion channels (LGICs, specifically the GABAA receptor). The reader is taken through a number of electrophysiological techniques applied to whole cell and excised patch recordings of recombinant and native GABAA receptor subtypes used in the generation and analysis of a variety of pharmacological parameters. These data interpretations form the basis for the analysis of potentially novel pharmacological agents active at the GABAA receptor target, but could equally be applied to other LGICs. The analysis is extended to incorporate post-synaptic inhibitory events in hippocampal neurons. Complementary descriptions of how tissues for such studies are prepared from recombinant and native receptor preparations are included. Attention is given to the physiological phenomena most relevant to everyday scientific literature.