UNIT 11.4 Use of Electrophysiological Methods in the Study of Recombinant and Native Neuronal Ligand-Gated Ion Channels
Published Online: 1 DEC 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Thomas, P. and Smart, T. G. 2012. Use of Electrophysiological Methods in the Study of Recombinant and Native Neuronal Ligand-Gated Ion Channels. Current Protocols in Pharmacology. 59:11.4:11.4.1–11.4.37.
- Published Online: 1 DEC 2012
Detailed in this unit are protocols for studying the effects of externally and internally applied agents on the behavior of ligand-gated ion channels (LGICs), specifically the GABAA receptor. These assays include a number of electrophysiological techniques applied to whole-cell and excised patch recordings of recombinant and native GABAA receptor subtypes used in the generation and analysis of a pharmacological data. Although applied to GABAA receptors, these techniques are equally applicable to other LGICs. The analysis is extended to incorporate consideration of post-synaptic inhibitory events. In addition, complementary descriptions of how tissues for such studies are prepared for studying recombinant and native receptors are included. Curr. Protoc. Pharmacol. 59:11.4.1-11.4.37. © 2012 by John Wiley & Sons, Inc.
- ligand-gated ion channels;
- tissue culture