Unit

UNIT 11.8 Electrophysiological Analysis of Tetrodotoxin-Resistant Sodium Channel Pharmacology

  1. Xiao-Wei Dong,
  2. Tony Priestley

Published Online: 1 FEB 2004

DOI: 10.1002/0471141755.ph1108s23

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Dong, X.-W. and Priestley, T. 2004. Electrophysiological Analysis of Tetrodotoxin-Resistant Sodium Channel Pharmacology. Current Protocols in Pharmacology. 23:11.8:11.8.1–11.8.33.

Author Information

  1. Schering-Plough Research Institute, Kenilworth, New Jersey

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: DEC 2003

Abstract

This unit describes essential procedures to record the activity of and the effects of pharmacological manipulation upon tetrodotoxin-resistant (TTXr) sodium channels. Readers are guided through the process of assembling a functional |patch-clamp| electrophysiology apparatus and construction of software-controlled voltage protocols that address various aspects of channel gating. Guidelines are also presented for analysis and display of the data typically generated by these experiments. For investigators wishing to study native channels in their appropriate cellular environment, protocols describing the isolation of viable cells from the main tissues that commonly express TTXr channels are also included.

Keywords:

  • sodium channel;
  • TTX-resistant;
  • activation;
  • inactivation;
  • state-dependent;
  • use-dependent;
  • voltage-dependent;
  • open channel;
  • inactivated channel;
  • tonic block