UNIT 11.14 Electrophysiological Studies of Voltage-Gated Sodium Channels Using QPatch HT, an Automated Patch-Clamp System
Published Online: 16 JUN 2014
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Liu, Y. 2014. Electrophysiological Studies of Voltage-Gated Sodium Channels Using QPatch HT, an Automated Patch-Clamp System. Current Protocols in Pharmacology. 65:11.14:11.14.1–11.14.45.
- Published Online: 16 JUN 2014
Voltage-gated sodium (Nav) channels are highly sensitive to membrane potential and have fast gating kinetics. Patch clamp electrophysiology has long been the gold standard for studying these channels. Combining high throughput with high information content/accuracy, automated patch clamp technologies have emerged as critical tools in ion channel drug discovery. Described in this unit is the use of QPatch, one of the automated patch clamp systems, to study Nav channel function and pharmacology. Curr. Protoc. Pharmacol. 65:11.14.1-11.14.45. © 2014 by John Wiley & Sons, Inc.
- voltage-gated sodium channel;
- Nav channel;
- patch clamp;