UNIT 12.8 Measurement of Cell Death in Mammalian Cells
Published Online: 1 SEP 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Cummings, B. S. and Schnellmann, R. G. 2004. Measurement of Cell Death in Mammalian Cells. Current Protocols in Pharmacology. 25:12.8:12.8.1–12.8.22.
- Published Online: 1 SEP 2004
- Published Print: JUN 2004
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This unit presents methods used to assess cell death in mammalian cells. The unit is divided into five sections: (1) a brief overview of cytotoxicity and pathways of cell death, (2) an improved method to measure cell death using lactate dehydrogenase (LDH) release as a marker of membrane integrity, (3) a flow cytometry method that simultaneously measures two types of cell death, oncosis and apoptosis, (4) use of nuclear morphology to assess apoptosis and oncosis, and (5) a brief discussion of the use of cytotoxicity assays to determine the mechanisms of cell death.