UNIT 12.9 Functional Screening in the Melanophore Bioassay

  1. Channa Jayawickreme,
  2. Howard Sauls,
  3. Chris Watson,
  4. David Moncol,
  5. Thomas Rimele,
  6. Terry Kenakin

Published Online: 1 JUL 2005

DOI: 10.1002/0471141755.ph1209s29

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Jayawickreme, C., Sauls, H., Watson, C., Moncol, D., Rimele, T. and Kenakin, T. 2005. Functional Screening in the Melanophore Bioassay. Current Protocols in Pharmacology. 29:12.9:12.9.1–12.9.16.

Author Information

  1. GlaxoSmithKline, Research Triangle Park, North Carolina

Publication History

  1. Published Online: 1 JUL 2005
  2. Published Print: JUN 2005


The melanophore bioassay is a robust, sensitive, and versatile procedure for screening G protein–coupled receptors in a variety of formats. Because melanophores contain a wide variety of G proteins, they can be employed as a sensitive, real-time response system for studying transfected receptors and for defining equilibria for drug effects. This assay can be run in 96-well microtiter plates or in open-lawn 1536 format, and can yield conventional agonist-antagonist as well as constitutive assays.


  • melanophore;
  • G-protein coupled receptors;
  • high throughput screening;
  • constitutive screening