UNIT 12.9 Functional Screening in the Melanophore Bioassay
Published Online: 1 JUL 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Jayawickreme, C., Sauls, H., Watson, C., Moncol, D., Rimele, T. and Kenakin, T. 2005. Functional Screening in the Melanophore Bioassay. Current Protocols in Pharmacology. 29:12.9:12.9.1–12.9.16.
- Published Online: 1 JUL 2005
- Published Print: JUN 2005
The melanophore bioassay is a robust, sensitive, and versatile procedure for screening G protein–coupled receptors in a variety of formats. Because melanophores contain a wide variety of G proteins, they can be employed as a sensitive, real-time response system for studying transfected receptors and for defining equilibria for drug effects. This assay can be run in 96-well microtiter plates or in open-lawn 1536 format, and can yield conventional agonist-antagonist as well as constitutive assays.
- G-protein coupled receptors;
- high throughput screening;
- constitutive screening