Unit

UNIT 12.11 Drug Testing in Cellular Chemotaxis Assays

  1. Mario Mellado,
  2. Carlos Martínez-A,
  3. José Miguel Rodríguez-Frade

Published Online: 1 JUN 2008

DOI: 10.1002/0471141755.ph1211s41

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Mellado, M., Martínez-A, C. and Rodríguez-Frade, J. M. 2008. Drug Testing in Cellular Chemotaxis Assays. Current Protocols in Pharmacology. 41:12.11:12.11.1–12.11.22.

Author Information

  1. Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Madrid, Spain

Publication History

  1. Published Online: 1 JUN 2008
  2. Published Print: JUN 2008

Abstract

Described in this unit are methods for measuring the cell migration process. While cell adhesion protocols allow study of migrating cell interactions with the endothelial matrix, cellular migration assays permit analysis of directed cell movement towards a chemotactic gradient, both in vivo and in vitro. An in vitro cell invasion protocol is provided for analysis of the sum of the cell adhesion, migration, and invasion activities involved in tumor cell motility. Curr. Protoc. Pharmacol. 41:12.11.1-12.11.22. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • adhesion;
  • migration;
  • chemokines;
  • integrins;
  • selectins