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UNIT 12.14 Measurement of Glucose Uptake in Cultured Cells

  1. Norio Yamamoto1,
  2. Manabu Ueda2,
  3. Takuya Sato1,
  4. Kengo Kawasaki1,
  5. Keisuke Sawada2,
  6. Kyuichi Kawabata2,3,
  7. Hitoshi Ashida2

Published Online: 1 DEC 2011

DOI: 10.1002/0471141755.ph1214s55

Lab Protocol Title

Current Protocols in Pharmacology

Current Protocols in Pharmacology

Additional Information

How to Cite

Yamamoto, N., Ueda, M., Sato, T., Kawasaki, K., Sawada, K., Kawabata, K. and Ashida, H. 2011. Measurement of Glucose Uptake in Cultured Cells. Current Protocols in Pharmacology. 55:12.14.1–12.14.22.

Author Information

  1. 1

    Food Science Research Center, House Wellness Foods Corporation, Hyogo, Japan

  2. 2

    Department of Agrobiosciences, Graduate School of Agricultural Science, Kobe University, Kobe, Japan

  3. 3

    Department of Bioscience, Fukui Prefectural University, Fukui, Japan

Publication History

  1. Published Online: 1 DEC 2011
  2. Published Print: DEC 2011
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Facilitative glucose uptake transport systems are ubiquitous in animal cells and responsible for transporting glucose across the cell surface membrane. Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders, such as myocardial ischemia, diabetes mellitus, and cancer. Methods for assessing glucose uptake into mammalian cells are detailed in this unit. The work is divided into four sections: (1) a brief overview of glucose uptake assays in cultured cells; (2) a method for measuring glucose uptake using radiolabeled 3-O-methylglucose; (3) a method for measuring glucose uptake using radiolabeled 2-deoxyglucose (2DG); and (4) an improved method for measuring 2DG-uptake using an enzymatic, fluorometric assay, eliminating the need for radiolabeled glucose analogs. Curr. Protoc. Pharmacol. 55:12.14.1-12.14.22. © 2011 by John Wiley & Sons, Inc.

Keywords: glucose uptake; cells; 3-O-methylglucose; 2-deoxyglucose; radioisotope; nonradioisotope

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