UNIT 12.14 Measurement of Glucose Uptake in Cultured Cells
Published Online: 1 DEC 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Yamamoto, N., Ueda, M., Sato, T., Kawasaki, K., Sawada, K., Kawabata, K. and Ashida, H. 2011. Measurement of Glucose Uptake in Cultured Cells. Current Protocols in Pharmacology. 55:12.14.1–12.14.22.
- Published Online: 1 DEC 2011
- Published Print: DEC 2011
Facilitative glucose uptake transport systems are ubiquitous in animal cells and responsible for transporting glucose across the cell surface membrane. Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders, such as myocardial ischemia, diabetes mellitus, and cancer. Methods for assessing glucose uptake into mammalian cells are detailed in this unit. The work is divided into four sections: (1) a brief overview of glucose uptake assays in cultured cells; (2) a method for measuring glucose uptake using radiolabeled 3-O-methylglucose; (3) a method for measuring glucose uptake using radiolabeled 2-deoxyglucose (2DG); and (4) an improved method for measuring 2DG-uptake using an enzymatic, fluorometric assay, eliminating the need for radiolabeled glucose analogs. Curr. Protoc. Pharmacol. 55:12.14.1-12.14.22. © 2011 by John Wiley & Sons, Inc.
- glucose uptake;