Unit

UNIT 13A.5 In Vivo Animal Models: Quantitative Models Used for Identifying Antibacterial Agents

  1. Jeffrey Fernandez

Published Online: 1 OCT 2006

DOI: 10.1002/0471141755.ph13a05s34

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Fernandez, J. 2006. In Vivo Animal Models: Quantitative Models Used for Identifying Antibacterial Agents. Current Protocols in Pharmacology. 34:A:13A.5:13A.5.1–13A.5.12.

Author Information

  1. Johnson & Johnson Pharmaceutical Research and Development, L.L.C., Raritan, New Jersey

Publication History

  1. Published Online: 1 OCT 2006
  2. Published Print: SEP 2006

Abstract

Determining the in vivo bactericidal potential of novel agents is critical for selecting drug candidates. Described in this unit are two protocols that measure the amount of bacterial killing under very different conditions. The mouse pouch protocol is a rapid assay that provides efficacy data in an acute infection, whereas the rabbit tissue cage protocol assesses the ability of a compound to eradicate bacteria in a chronic abscess infection. Data from these tests, along with those from other qualitative and quantitative assays, are important for defining antibacterial efficacy in vivo.

Keywords:

  • abscess;
  • subcutaneous infection;
  • tissue cage infection;
  • mouse pouch