UNIT 13A.6 In Vitro Antibacterial Resistance Selection and Quantitation

  1. Katherine Young

Published Online: 1 OCT 2006

DOI: 10.1002/0471141755.ph13a06s34

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Young, K. 2006. In Vitro Antibacterial Resistance Selection and Quantitation. Current Protocols in Pharmacology. 34:A:13A.6:13A.6.1–13A.6.22.

Author Information

  1. Merck Research Laboratories, Rahway, New Jersey

Publication History

  1. Published Online: 1 OCT 2006
  2. Published Print: SEP 2006


The study of resistance is vital to the discovery and development of new antibacterial agents. Consider the case where the resistance frequency (the proportion of cells within a population that exhibit a resistance phenotype to the agent under study) of an agent is such that, in an average infection, a mutant resistant to the agent is likely to already exist. This compound will not be useful in the clinic as a single agent, since this mutant can survive its administration, leading to potential clinical failure. This unit provides protocols for determining the frequency and rate of resistance to antibacterial agents, as well as generating resistant mutants for mechanism of action determinations or for use in antibacterial discovery or development programs.


  • growth curve;
  • agar-dilution MIC;
  • resistance frequency;
  • resistance rate;
  • fluctuation test