UNIT 13A.8 Static Biofilm Cultures of Gram-Positive Pathogens Grown in a Microtiter Format Used for Anti-Biofilm Drug Discovery
Published Online: 1 SEP 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Kwasny, S. M. and Opperman, T. J. 2010. Static Biofilm Cultures of Gram-Positive Pathogens Grown in a Microtiter Format Used for Anti-Biofilm Drug Discovery. Current Protocols in Pharmacology. 50:A:13A.8:13A.8.1–13A.8.23.
- Published Online: 1 SEP 2010
- Published Print: SEP 2010
An in vitro assay is presented for culturing staphylococcal biofilms and biofilms of nonmotile Gram-positive bacteria under static conditions in microtiter assay plates, and for the quantification of biofilm growth, using a simple staining procedure that measures amounts of bacterial cells and extracellular matrix. This basic assay can be adapted readily to study several aspects of biofilm formation, for high-throughput screening to identify small molecule inhibitors of biofilm formation or biofilm-defective mutants, and for quantifying the anti-biofilm activity of biofilm inhibitors. Curr. Protoc. Pharmacol. 50:13A.8.1-13A.8.23. © 2010 by John Wiley & Sons, Inc.