Unit

UNIT 13A.8 Static Biofilm Cultures of Gram-Positive Pathogens Grown in a Microtiter Format Used for Anti-Biofilm Drug Discovery

  1. Steven M. Kwasny,
  2. Timothy J. Opperman

Published Online: 1 SEP 2010

DOI: 10.1002/0471141755.ph13a08s50

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Kwasny, S. M. and Opperman, T. J. 2010. Static Biofilm Cultures of Gram-Positive Pathogens Grown in a Microtiter Format Used for Anti-Biofilm Drug Discovery. Current Protocols in Pharmacology. 50:A:13A.8:13A.8.1–13A.8.23.

Author Information

  1. Microbiotix, Anti-Infectives R&D, Worcester, Massachusetts

Publication History

  1. Published Online: 1 SEP 2010
  2. Published Print: SEP 2010

Abstract

An in vitro assay is presented for culturing staphylococcal biofilms and biofilms of nonmotile Gram-positive bacteria under static conditions in microtiter assay plates, and for the quantification of biofilm growth, using a simple staining procedure that measures amounts of bacterial cells and extracellular matrix. This basic assay can be adapted readily to study several aspects of biofilm formation, for high-throughput screening to identify small molecule inhibitors of biofilm formation or biofilm-defective mutants, and for quantifying the anti-biofilm activity of biofilm inhibitors. Curr. Protoc. Pharmacol. 50:13A.8.1-13A.8.23. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • biofilm;
  • microtiter;
  • Gram-positive