UNIT 13B.6 Preparation of HCV NS3 and NS5B Proteins to Support Small-Molecule Drug Discovery
Published Online: 1 SEP 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Hung, M., Wang, R. and Liu, X. 2011. Preparation of HCV NS3 and NS5B Proteins to Support Small-Molecule Drug Discovery. Current Protocols in Pharmacology. 54:B:13B.6:13B.6.1–13B.6.18.
- Published Online: 1 SEP 2011
- Published Print: SEP 2011
Production of high-quality, well-characterized recombinant proteins facilitates screening of compound libraries. The protocols detailed in this unit are used to purify three recombinant enzymes that are widely used in HCV research: the HCV NS3 protease domain, the helicase domain as an NS3+NS4A complex, and the NS5B RNA-dependent RNA polymerase. The active enzymes are purified to homogeneity by two-column chromatography to support a screening program for HCV inhibitors. Curr. Protoc. Pharmacol. 54:13B.6.1-13B.6.18. © 2011 by John Wiley & Sons, Inc.
- protein purification