Unit

UNIT 14.3 Orthotopic Model of Human Pancreatic Ductal Adenocarcinoma and Cancer Cachexia in Nude Mice

  1. Susan Jones-Bolin,
  2. Bruce Ruggeri

Published Online: 1 JUN 2007

DOI: 10.1002/0471141755.ph1403s37

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Jones-Bolin, S. and Ruggeri, B. 2007. Orthotopic Model of Human Pancreatic Ductal Adenocarcinoma and Cancer Cachexia in Nude Mice. Current Protocols in Pharmacology. 37:14.3:14.3.1–14.3.21.

Author Information

  1. Cephalon, Inc., West Chester, Pennsylvania

Publication History

  1. Published Online: 1 JUN 2007
  2. Published Print: JUN 2007

Abstract

Pancreatic ductal adenocarcinoma (PDAC) represents the fourth leading cause of cancer-related deaths in the United States, with a 5-year survival rate of only 2% to 10%. This tumor is aggressive, often metastasizing to distant sites (liver, lung, and adjacent intestines) by the time of diagnosis. Treatment options are limited, and the disease carries a grave prognosis for most patients. An orthotopic model of human PDAC in nude mice provides an excellent way to evaluate the pathogenesis of tumor growth and metastasis in order to develop therapies, to better define the underlying biology of tumor growth and metastasis, and to identify new molecular targets. This unit describes an orthotopic model of human PDAC in athymic nude mice that closely mimics the human condition. It is characterized by diffuse peritoneal, lymphatic, and hepatic metastatic spread and manifestations of a cancer cachexic phenotype.

Keywords:

  • Human pancreatic ductal adenocarcinoma;
  • Athymic nude mice;
  • Orthotopic;
  • Metastasis;
  • cachexia