Unit

UNIT 14.16 Establishment, Maintenance, and In Vitro and In Vivo Applications of Primary Human Glioblastoma Multiforme (GBM) Xenograft Models for Translational Biology Studies and Drug Discovery

  1. Brett L. Carlson,
  2. Jenny L. Pokorny,
  3. Mark A. Schroeder,
  4. Jann N. Sarkaria

Published Online: 1 MAR 2011

DOI: 10.1002/0471141755.ph1416s52

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Carlson, B. L., Pokorny, J. L., Schroeder, M. A. and Sarkaria, J. N. 2011. Establishment, Maintenance, and In Vitro and In Vivo Applications of Primary Human Glioblastoma Multiforme (GBM) Xenograft Models for Translational Biology Studies and Drug Discovery. Current Protocols in Pharmacology. 52:14.16:14.16.1–14.16.23.

Author Information

  1. Mayo Clinic, Department of Radiation Oncology, Rochester, Minnesota

Publication History

  1. Published Online: 1 MAR 2011
  2. Published Print: MAR 2011

Abstract

Development of clinically relevant tumor model systems for glioblastoma multiforme (GBM) is important for advancement of basic and translational biology. One model that has gained wide acceptance in the neuro-oncology community is the primary xenograft model. This model entails the engraftment of patient tumor specimens into the flank of nude mice and subsequent serial passage of these tumors in the flank of mice. These tumors are then used to establish short-term explant cultures or intracranial xenografts. This unit describes detailed procedures for establishment, maintenance, and utilization of a primary GBM xenograft panel for the purpose of using them as tumor models for basic or translational studies. Curr. Protoc. Pharmacol. 52:14.16.1-14.16.23. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • glioblastoma;
  • xenograft;
  • mouse models