Unit

UNIT 14.27 Utility and Applications of Orthotopic Models of Human Non-Small Cell Lung Cancer (NSCLC) for the Evaluation of Novel and Emerging Cancer Therapeutics

  1. Verline Justilien,
  2. Alan P. Fields

Published Online: 8 OCT 2013

DOI: 10.1002/0471141755.ph1427s62

Current Protocols in Pharmacology

Current Protocols in Pharmacology

How to Cite

Justilien, V. and Fields, A. P. 2013. Utility and Applications of Orthotopic Models of Human Non-Small Cell Lung Cancer (NSCLC) for the Evaluation of Novel and Emerging Cancer Therapeutics. Current Protocols in Pharmacology. 62:14.27:14.27.1–14.27.17.

Author Information

  1. Department of Cancer Biology, Mayo Clinic College of Medicine, Jacksonville, Florida

Publication History

  1. Published Online: 8 OCT 2013

Abstract

Lung cancer is a leading cause of cancer deaths worldwide. Despite advances in chemotherapy, radiation therapy, and surgery, lung cancer continues to have a low 5-year survival rate, highlighting a dire need for more effective means of prevention, diagnosis, prognosis, and treatment. Mouse models that recapitulate the clinical features of advanced human lung cancer are critical for testing novel therapeutic approaches. This unit describes a highly reproducible, easy-to-establish orthotopic murine model of lung cancer, provides methods for in vivo imaging and monitoring of tumor growth, and discusses the usefulness of this model for translational lung cancer research and the development of therapeutic strategies. Curr. Protoc. Pharmacol. 62:14.27.1-14.27.17. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • non-small cell lung cancer;
  • orthotopic model;
  • bioluminescent in vivo imaging