UNIT 14.28 Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors—Potential Applications
Published Online: 2 DEC 2013
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Pharmacology
How to Cite
Dobbin, Z. C. and Landen, C. N. 2013. Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors—Potential Applications. Current Protocols in Pharmacology. 63:14.28:14.28.1–14.28.19.
- Published Online: 2 DEC 2013
Cancer stem cells (CSCs) are a subpopulation of cells within a heterogeneous tumor that have enhanced biologic properties, e.g., increased capacity for self-renewal, increased tumorigenicity, enhanced differentiation capacity, and resistance to chemo- and radiotherapies. This unit describes protocols to isolate and characterize potential cancer stem cells from a solid tumor. These involve creating a single-cell suspension from tumor tissue, tagging the cell subpopulations of interest, and sorting them into different populations. The sorted subpopulations can be evaluated for their ability to meet the functional requirements of a CSC, which primarily include increased tumorigenicity in an in vivo xenograft assay. Use of the protocols described in this unit makes it possible to study populations of cells that may have properties of CSCs. Curr. Protoc. Pharmacol. 63:14.28.1-14.28.19. © 2013 by John Wiley & Sons, Inc.
- cancer stem cells;
- xenograft assay;
- cell separation and sorting